Influence of forest vegetation restoration on carbon increment after mining

We have clarified the study area has a history of 65 years and has been restored for 6 years. This study investigated the carbon storage characteristics of undisturbed natural forests and restored mining vegetation in Yunnan Province, China. The goal was to quantify carbon reserves and increments to inform ecological restoration strategies. Four vegetation components (tree, shrub, herb, litter) and five soil layers (0–10, 10–20, 20–30, 30–40, 40–60 cm) were analyzed. In natural forest, the tree layer stored 60% of carbon (273 Mg ha−1), overwhelmingly dominating vegetation carbon stocks. Shrub, herb, and litter layers each comprised < 1%. Surface soil layers (0–30 cm) stored 64% of soil carbon. In the restored mining area, the tree layer contributed 75% of vegetation carbon increment (16 Mg ha−1), though stocks were lower than natural forest. Soil layers showed the highest carbon increment (69%) despite lower biomass than natural conditions. Unexploited forests thus exhibit robust carbon storage, while restored mining areas have weaker carbon gains, indicating recovery potential. Strategic interventions targeting soil quality, stimulating vegetation growth, and increasing carbon sequestration could significantly augment reserves and ecological functionality. Prioritizing vegetation succession and soil revitalization are paramount to ensuring ecological integrity and sustainable development. Fostering a positive regional ecological feedback loop will be pivotal. This research quantifies carbon storage differences between undisturbed and restored mining areas, highlighting soil and vegetation as critical targets for optimizing carbon sequestration and ecosystem recovery in degraded environments.


Overview of the study area
The mining study area is located in the Shan Zhuang and Miao Autonomous Prefecture of Yunnan Province, China (Fig. 1).The roads leading to the site are national secondary and tertiary roads and are fully asphalted, allowing for easy transport.Mining operations began in the 1970s and the study site has undergone vegetation restoration.The primary vegetation types of the site include subtropical evergreen deciduous forest, mixed coniferous forest and deciduous coniferous forest dominated by fir (Cunninghamia lanceolata) and kai (Alnus cremastogyne Burk).The topography is complex, with significant height differences, low forest cover and a single species structure of timber and charcoal forest dominated by fir.Forest and grass cover in the project area is 34.24% and the primary soil-forming parent materials include limestone, Quaternary red clay and recent sediment.Soil survey data show that the project area is divided into six soil types, 12 subsoils, 16 soil genera and 19 representative soil species, dominated by red clay, red soil, limestone (rock) soil and rice soil.

Sample plot set-up
The carbon stocks of the natural forest vegetation in the vicinity of the mining area are measured to assess the carbon storage in the natural forest vegetation of the mining area under natural conditions.The objective of the experiment was to measure carbon increment by assessing the carbon stock of forest vegetation under natural conditions around the mine site.To ensure consistency between the experimental sample plots, three standard sample plots were established, each measuring 20 m x 20 m.Factors such as climate, vegetation, topography, site conditions and mine density were considered in the selection of sample plots.In addition, five 5 m x 5 m shrub thicket plots, five 1 m x 1 m herbaceous community plots and three 1 m x 1 m litters plots were established along the diagonal of each sample plot.These were used to collect data on shrubs, herbs and litters.Table 1 shows the basic conditions of natural vegetation samples around the mining area.
Three standard plots with an area of 20 m × 20 m were established within the mining area to measure the carbon increment in vegetation and soil after vegetation restoration.Along the diagonal direction of each plot, five shrub plots with an area of 5 m × 5 m and five herbaceous community plots with an area of 1 m × 1 m were set up.Additionally, three litter plots with an area of 1 m × 1 m were established to collect samples of shrubs, herbaceous plants, and litter.Table 2 shows the basic situation of vegetation restoration samples in the mining area.

Sample collection
All samples (natural or mine) are handled separately once they are sent to the laboratory and plant and soil samples are not mixed to avoid confusion between samples.
(1) Collection of plant samples.Collect different organs (leaves, branches, stems, bark and roots), shrub layers (leaves, roots, branches) and herb layers (above and below ground) of typical standard trees in each sampling area.Collect 500 g of each characteristic plant part separately and weigh them in the field.At the same time, take photographs of the characteristics and growth of each plant, such as flowers, leaves, fruits, etc.Based on the Flora of China and related works and literature, accurately identify the species and genera of wild plants and conduct field surveys.Record the number of layers of trees, species names, tree heights and diameter at breast height; record the species names, number of plants (clumps) and height of layers of shrubs and herbs, and estimate the coverage; litter according to its contact distance with the ground.
The process of litter collection must be carried out carefully to maintain the integrity and accuracy of the sample.Litter is usually divided into three levels of decomposition: undecomposed layer, semi-decomposed layer and decomposed layer.Undecomposed layer usually refers to the surface litter, mainly containing leaves, twigs and other substances that have not significantly decomposed.Semi-decomposed layer is a layer between the undecomposed layer and the decomposed layer, containing some decomposed but not completely degraded litter.The decomposed layer is the layer of litter that is clearly decomposed and incorporated into the soil 14 .The following steps should be followed when collecting litter: use a small shovel or similar tool and carefully remove different decomposition layers in the soil to ensure that the samples obtained are representative.Finally, during the collection process, attention should be paid to the degree of decomposition of the litter to ensure that the samples belong to different decomposition layers.The collected materials of different decomposition layers should be placed in bags or containers marked with the sampling point information to ensure that the identification of the samples is clear and accurate.During the collection process, it is necessary to keep the sample dry and clean to avoid mixing of foreign materials.In addition, the location and date of sampling are recorded to ensure that subsequent analysis is accurate.
At the same time, the collected samples of trees, shrubs, herbs and litter were sent back to the laboratory and dried at 65 ℃ to constant weight to calculate biomass.
In each sample, 5 soil profiles are excavated and 5 mixed soil samples are taken from different depths.The proportion of gravel in the soil layer below 60 cm is relatively high, and it is not in the soil layer.The soil is divided into 5 layers, namely 0-10, 10-20, 20-30, 30-40 and 40-60 cm, with approximately 500 g of soil sample collected from each layer.For each soil layer profile, a 100 cm 3 ring knife is required to take soil samples, and The sample is cleaned of small debris and other contaminants and then returned to the laboratory for natural air drying.The soil sample is then ground and sieved (100 mesh) for the determination of soil organic carbon 15 .

Calculation of carbon content and carbon increment of sample plots
The carbon storage of natural forest vegetation in the mining area was measured based on the carbon storage of forest vegetation under natural conditions surrounding the mining area.The carbon increment in the mining area was measured based on the carbon storage of vegetation and soil after vegetation restoration in the mining area.Determination of organic carbon content in plant and soil samples using potassium dichromate volumetric method in the laboratory 16 : In the equation, VCS represents the carbon storage and carbon increment of the vegetation layer.(Mg ha −1 ), OC is the organic carbon content (%) and B is the biomass per unit area (Mg ha −1 ).
In the equation, TN represents the total soil carbon storage and carbon increment within the depth profile of the soil layer.(g•cm −3 ) in the layer soil profile depth; CN is the soil carbon content (%) in layer N; PN is the soil capacity (g•cm −3 ) in layer N: DN is the layer N profile depth.

Analytical processing of data
One-way ANOVA was used to test the variability of carbon increments in each stand, and Excel 2013 and SPSS 20.0 were used to process the graphs and carry out the data analysis.The map in Fig. 1 was generated using ArcGIS Desktop 10.7 (Esri, Redlands, CA, USA).ArcGIS is a geographic information system (GIS) software that allows for mapping and spatial analysis of data.Version 10.7 was used to create the map by importing and georeferencing the relevant datasets, applying symbology, and exporting the map as an image file for inclusion in the article.The latest version of ArcGIS Desktop can be downloaded from https:// www.esri.com/ en-us/ arcgis/ produ cts/ arcgis-deskt op/ overv iew.

Carbon content, biomass and carbon stock characteristics of the tree layer
According to Table 3, the carbon content in the tree layer shows the following order: stem > leaf > bark > root > branch, ranging from 48.05% to 58.76%.The biomass of the tree layer is highest in the stem (198.76Mg ha −1 ), followed by the branch (143.04Mg ha −1 ) and the root (73.12 Mg ha −1 ), while the leaf (51.21 Mg ha −1 ) and the bark (28.28 Mg ha −1 ) have the lowest biomass.Carbon storage in the stem accounts for the majority of carbon storage in the tree layer, representing 45% of the total, followed by the branch (25%) and root (12%), while the leaf (10%) and bark (5%) have the smallest contributions.The order of carbon storage is stem > branch > root > leaf > bark.

Carbon content, biomass and carbon stock characteristics of shrub layer
Based on the carbon content of the shrub layer (Table 4), the order from highest to lowest is branch > root > leaf.Among the organs of the shrub layer, there is no significant difference between the root and the branch (P > 0.05), while the leaf shows a significant difference from the root and the branch (P < 0.05).The biomass distribution of the different organs in the shrub layer is consistent with the carbon content.The branch has the highest biomass in the shrub layer with 44.7% of the total biomass, followed by the root (34.7%) and the leaf has the lowest biomass (20.6%).The order is branch > root > leaf.
When comparing different vegetation types within the same shrub layer, there are differences in biomass and significant differences between organs (P < 0.05).The main organ contributing to the carbon storage in the shrub layer is the branch, which accounts for 54.0% of the carbon storage in the shrub layer, followed by the root (30.7%), and the leaf has the smallest contribution (16.3%).The order is: branch > root > leaf.
(1) The below ground part of the herbaceous layer accounts for 55.1% of the carbon content, followed by the above ground part (44.9%).The carbon content is higher in the belowground part than in the aboveground part (Table 5).The biomass of the aboveground part of the herbaceous layer (10.15 Mg ha −1 ) is higher than that of the belowground part (7.10 Mg ha −1 ).Similarly, the above-ground portion contributes to 57.6% of the carbon storage in the herbaceous layer, followed by the below-ground portion (42.4%).

Carbon content, biomass and carbon stock characteristics of the litters layer
The carbon content in the litter layer shows a significant difference (P < 0.05) between the different decomposition stages, with the undecomposed layer having the highest carbon content, followed by the partially decomposed layer and the fully decomposed layer.The carbon content decreases with increasing degree of decomposition (Table 6).The biomass distribution in the litter layer is highest in the fully decomposed layer (6.96 Mg ha −1 ), followed by the partially decomposed layer (3.59 Mg ha −1 ), and lowest in the undecomposed layer (1.24 Mg ha −1 ), indicating a pattern of fully decomposed layer > partially decomposed layer > undecomposed layer, which shows significant differences (P < 0.05).In terms of carbon storage in the litter layer, the partially decomposed layer has the highest carbon storage, accounting for 63.9% of the carbon storage in the litter layer, followed by the partially decomposed layer (24.5%), and the fully decomposed layer has the lowest carbon storage (11.6%).

Carbon content, biomass and carbon stock characteristics of soil layers
The soil carbon content at the three sampling sites decreases significantly with increasing soil depth (P < 0.05).
The distribution of soil carbon content varies between different soil layers, with the topsoil layer (0 -1 0 cm) having the highest carbon content and the 40-60 cm soil layer having the lowest carbon content (Table 7).The factors

Carbon content, biomass and carbon stock characteristics of natural vegetation
The carbon content of the different components in natural vegetation follows the order: tree layer > soil layer > litter layer > herb layer > shrub layer (Table 8).In natural vegetation, the main carbon storage component is the tree layer, which accounts for 60% of the carbon storage in natural vegetation.The soil layer contributes 37% of the carbon storage, while the shrub layer (0.6%), the herbaceous layer (0.6%) and the litter layer (0.7%) have relatively small shares in carbon storage.

Carbon content, biomass and carbon increment characteristics of the tree layer
Table 9 shows that within the tree layer the carbon content followed a descending order: leaf > root > branc h > bark > stem.The highest biomass in the tree layer was found in roots (45.00 Mg ha −1 ), followed by leaves (40.91 Mg ha −1 ) and stems (31.33 Mg ha −1 ), while the lowest biomass was found in peels (29.66 Mg ha −1 ) and branches (26.36 Mg ha −1 ).Leaves were responsible for the largest carbon increment in the tree layer, contributing 47.3% of the total, followed by roots (25.1%) and branches (11.6%).The smallest carbon increment came from the stem (8.8%) and bark (7.2%).In summary, the order of carbon accumulation in the tree layer was: leaves > roots > branches > stem > bark.

Carbon content, biomass and carbon increment characteristics of the shrub layer
As for the shrub layer (Table 10), branches had the highest carbon content, followed by roots and leaves, which differed significantly from each other (P < 0.05).Branches also accounted for the largest part of the shrub layer biomass (43.3%), followed by leaves (29.5%) and roots (29.2%).The biomass distribution of the shrub layer organs differed significantly (P < 0.05), in the order: branches > leaves > roots.Branches were also responsible for the highest carbon increment in the shrub layer, contributing 59.1% of the total, followed by roots (24.3%) and leaves (16.6%).Therefore the order of carbon increment in the shrub layer was: branches > roots > leaves.www.nature.com/scientificreports/

Carbon content, biomass and carbon increment characteristics of the herbaceous layer
In the herbaceous layer (Table 11), the carbon content was higher in the above-ground part than in the belowground part, with shares of 55.3% and 44.7%, respectively.However, biomass was higher in the below-ground part (0.54 Mg ha −1 ) than in the above-ground part (0.26 Mg ha −1 ).Similarly, the carbon increment in the belowground part accounted for 66.7% of the herbaceous layer, while the above-ground part contributed 33.3%.

Characteristics of carbon content, biomass and carbon increment in the litters layer
Regarding the litters layer (Table 12), the carbon content decreased with the decomposition of the material in the following order: undecomposed layer > semi-decomposed layer > decomposed layer.The carbon content of the different organs also differed significantly (P < 0.05).The biomass distribution of the litters layer was opposite to the carbon content, with the highest biomass found in the decomposed layer (1.76 Mg ha −1 ), followed by the semi-decomposed layer (1.22 Mg ha −1 ) and the lowest biomass in the undecomposed layer (0.98 Mg ha −1 ).In terms of carbon increment, the highest contribution came from the decomposed layer (40.0%), followed by the semi-decomposed layer (31.4%), and the lowest from the undecomposed layer (28.6%).Therefore, the order of carbon increment in the litters layer was: decomposed layer > semi-decomposed layer > undecomposed layer.

Soil layer carbon content, biomass and carbon increment characteristics
Soil carbon content was found to be strongly influenced by soil depth, with a significant decrease (P < 0.05) observed with increasing depth.Carbon content was unevenly distributed among the soil layers, with the highest concentration found in the topsoil layer (0-10 cm) and the lowest in the 40-60 cm layer (Table 13).In contrast, soil bulk density increased with depth, and the bulk density of the 40-60 cm layer was 3.8 times higher than that of the 0-10 cm layer.Both soil carbon content and capacity affect soil carbon increment, which also decreases with increasing soil depth, consistent with the trend observed for soil carbon content.The top 30 cm of soil accounted for 69.5% of total soil carbon increment, indicating that most of the carbon increment in the soil layer was concentrated in the topsoil.

Ecosystem carbon content, biomass and carbon increment characteristics
In the vegetation layer, carbon increment is determined by both the carbon content and biomass of each component, with higher carbon content and biomass leading to higher carbon increment.In the forest ecosystem, the carbon content of each component followed the order tree layer > soil layer > litters layer > herb layer > shrub layer (Table 14).Biomass (capacity) was highest in the tree layer (161.58Mg ha −1 ), followed by the shrub layer (6.89 Mg ha −1 ), coppice layer (0.35 Mg ha −1 ), soil layer (2.95 Mg ha −1 ) and herb layer (0.03 Mg ha −1 ).The lowest www.nature.com/scientificreports/carbon accumulation in the forest ecosystem was observed in the soil layer (2.95 Mg ha −1 ) and herb layer (0.03 Mg ha −1 ), indicating that the order of carbon accumulation was tree layer > shrub layer > litters layer > soil layer > herb layer.The tree layer was found to be the main contributor to carbon increment in the forest ecosystem, accounting for 75.1% of the total, followed by the soil layer (16.5%), shrub layer (5.2%) and litters layer (1.6%), with the herbaceous layer contributing the least (0.01%).

Carbon stock characteristics of natural vegetation in the mining area
This study focused on estimating the carbon stock of natural vegetation in mining areas.We selected representative undisturbed natural vegetation near mining sites to investigate the carbon storage within the area.The natural vegetation was categorized into four components: the tree layer, shrub layer, herb layer, and litter layer 17 .Carbon content varied among these vegetation types, and we determined the carbon storage by measuring carbon content and biomass, which improved the accuracy of our calculations.Biomass was the primary form of carbon accumulation in natural vegetation, influenced by regional climate, soil types, forest vegetation, and plant age 18 .Carbon storage in the tree layer dominated the overall carbon storage in the vegetation layer of the mining area, accounting for 94% of the vegetation layer and 60% of all natural vegetation.However, carbon loss from vegetation was influenced not only by vegetation composition and tree age but also by factors such as regional climate, ambient light, surface types, and cavity composition 19 .Within the tree layer, carbon storage followed the order: roots > branches > stems > leaves > bark, with leaves and bark having lower carbon storage due to tissue senescence and slower carbon cycling.
The shrub layer contributed 3% of the carbon stored in natural vegetation, significantly more than the herb and litter layers.In the herb layer, aboveground carbon storage exceeded belowground carbon storage.The litter layer played an important role in the carbon input from the forest to the soil 20,21 , with the partially decomposed layer having the highest carbon storage among the different litter components.The soil organic carbon content varied among the different soil layers, with the topsoil (0-10 cm) having the highest content and the 40-60 cm layer having the lowest.Long-term use of the mining area resulted in severe damage to vegetation and soil layers.In an undisturbed scenario, soil carbon storage would have been higher due to the presence of shrubs and litter layers, which contribute to carbon replenishment through decomposition.In addition, slow decomposition of organic matter and the presence of rich community vegetation played a role in maintaining higher soil carbon storage in the 0-30 cm soil layer, which accounted for 64% of total soil carbon storage.
The main factors influencing carbon storage in natural vegetation in mining areas were plot type, vegetation density and forest self-regulation practices.The forest ecosystem in the mining area showed higher carbon storage compared to the average of Chinese forest ecosystems (258.8Mg ha −1 ) 22 , indicating a strong carbon sequestration potential.Therefore, it is essential to implement protection measures, improve soil quality and increase carbon reserves in the vegetation layer to restore the ecological integrity and land productivity of degraded mining areas.

Characteristics of carbon increment in vegetation restoration in mining areas
Mining activities have brought both benefits and negative environmental impacts, such as soil erosion and carbon loss.To address these issues, this study aimed to determine the carbon increment following vegetation restoration in mining areas 23 .We selected an undisturbed forest ecosystem adjacent to the mining site as a research object to guide the selection of tree species and ecological restoration models for the mine site.Carbon content varies between different organ types and we used measured carbon content and biomass to more accurately calculate carbon increment.Biomass accumulation is the primary form of plant growth in forest ecosystems and is influenced by regional climate, soil type, forest vegetation type and plant age 24 .Biomass in the mining area was significantly lower than that in undisturbed natural forest ecosystems (552.8Mg ha −1 ), indicating significant potential for improvement.
Vegetation recovery and succession are complex processes influenced by vegetation-soil interactions, with different vegetation types having different biomass and carbon content due to their physiological and ecological characteristics 25,26 .Soil properties also play an important role in soil organic carbon cycling.The distribution of carbon increment differs among the organic matter types, with the soil layer showing the highest carbon increment, followed by the tree layer, shrub layer, litters layer and herb layer.Studies consistently show that organic carbon content decreases significantly with increasing soil depth 27 .In our study, carbon increment in the 0-30 cm soil layer accounted for 69.3% of total soil carbon increment 28,29 , exceeding the global average for total soil carbon increment in all soil surface layers.This suggests that carbon increment in the surface layer was the major contributor to soil carbon increment in our experiment.

Figure 1 .
Figure 1.Geographic location of the mine and sampling sites.

Table 1 .
Sample plot layout of natural vegetation around the mining area Natural vegetation around the mining area.N stands for north direction.

Table 2 .
Layout of vegetation plots in the mining area.

Table 4 .
Carbon content, biomass and carbon loss of shrub layer.

Table 5 .
Carbon content, biomass and carbon storage of herbaceous layer.

Table 6 .
Carbon content, biomass and carbon loss reserves of litter layer.

Table 7 .
Carbon content, biomass and carbon loss characteristics of soil layer.influencingsoil carbon storage are soil carbon content and bulk density.As soil carbon storage increases, soil depth decreases and the magnitude of the change is generally consistent with soil carbon content.Soil carbon storage in the 0-30 cm soil layer accounts for 64.2% of total soil carbon storage, indicating that the majority of soil carbon storage is concentrated in the surface soil layer.

Table 8 .
Carbon content, biomass and carbon loss reserves of natural vegetation.

Table 9 .
Carbon content, biomass and carbon increment in the tree layer.

Table 10 .
Carbon content, biomass and carbon increment in the shrub layer.

Table 11 .
Carbon content, biomass and carbon gain in the herbaceous layer.

Table 12 .
Carbon content, biomass and carbon increment in the litters layer.

Table 13 .
Soil layer carbon content, biomass and carbon increment.

Table 14 .
Ecosystem carbon content, biomass and carbon increment.